CS/B.Sc.(H)/BT/GEN/MICRO-BIO/MOL-BIO/SEM-4/MOG-401/2013
2013
MOLECULAR GENETICS
Time Allotted : 3 Hours Full Marks : 70
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words
as far as practicable.
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following :
10x1 = 10
i) Which of the following methods is most useful for
enzymatic amplification of specific segment of DNA ?
a) Nucleotide sequencing
b) DNA hybridization
c) PCR
d) None of these.
ii) Gene expression is analyzed by
a) Southern blot b) Restriction digestion
c) Northern blot d) None of these.
iii) In sourthern blotting experiment, the binding of
transferred DNA to the nylon membrane is ……………
type.
a) ionic b) covalent
c) hydrophobic d) van der Waals.
iv) DNA hybridization is a technique, which relies on the
following properties of DNA, except
a) Double strandedness
b) sequence specificity
c) major and minor grooves
d) denaturation-renaturation properties.
v) For RNA detection which PCR is best ?
a) Nested PCR b) RT-PCR
c) ARMS-PCR d) MS-PCR.